B-Cell Linker anticorps (pTyr84)

N° du produit ABIN967596

Cet anticorps anti-B-Cell Linker est un anticorps Souris Monoclonal détectant B-Cell Linker dans WB. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour B-Cell Linker anticorps (pTyr84) (ABIN967596)

Antigène: B-Cell Linker (BLNK)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp B-Cell Linker est non-conjugé

Application: Western Blotting (WB)

Clone: J117-1278

Détail du produit anti-B-Cell Linker anticorps

Épitope: pTyr84

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human BLNK Peptide

Isotype: IgG2b kappa

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-B-Cell Linker (ABIN967596)

Pighi, Gu, Dalai, Barbi, Parolini, Bertolaso, Pedron, Parisi, Ren, Cecconi, Chilosi, Menestrina, Zamò: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." dans: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, (2011) (PubMed).

Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." dans: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." dans: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." dans: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

Chiu, Dalton, Ishiai, Kurosaki, Chan: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." dans: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, (2002) (PubMed).

Détails sur B-Cell Linker

Antigène: B-Cell Linker (BLNK)

Autre désignation: BLNK

Sujet: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Poids moléculaire: 68 kDa

Pathways: BCR Signaling